Of these, only 17 isolates belonged to Lactobacillus genus (Table (Table1 1). Some microorganisms such as Lactobacillus plantarum, Lactobacillus casei, Escherichia coli C162, Saccharomyces cerevisiae, and Kloeckera brevis require biotin for growth and can be used for measuring biotin. Finally, the single colony of Lactobacillus was isolated by observing their colony morphology and some biochemical tests such as gram staining, catalase and oxidase test. 37 These microbial methods are the first methods used for measuring biotin but now there are other more specific methods for biotin measurements.
By performing morphological and biochemical tests, the rod- or spherical-shaped gram-positive and catalase-negative single colonies that could belong to the Lactobacillus genus were isolated. Some of the characteristics are as follows: Fermentation of (For L. acidophilus) Biochemical Test of Lactobacillus spp. A total of 125 bacteria were isolated from yogurt and colostrum.
Classical phenotypic tests for identification of lactobacilli are based on physiological characteristics, like motility, growth temperature, respiratory type and growth in sodium chloride, as well as on diverse biochemical characteristics, such as fermentation type, metabolism of carbohydrate substrates, production of lactic acid isomers, coagulation of milk and presence of specific enzymes like arginine … The proteins avidin and streptavidin specifically …
Well isolated colonies were picked up and transferred to MRS broth for enrichment of Lactobacillus at 37°C.
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